Temperature dependency of immunoglobulin production in novel human partner cell line

Introduction A number of immunoglobulin (Ig) secreting human hybrid cell lines were created using one-on-one somatic cell hybridization of a rare human tumor infiltrating B lymphocyte and a cell of a novel human cell line (WTM), developed in house and described earlier [1]. These hybrid cell lines secret various amounts of tumor-derived immunoglobulins (Igs) of different specificities. Current investigative efforts are directed towards determining the optimal culture conditions to ensure consistent cell growth and long-term stabilities of Ig productions by the hybrids. Based on previous literature reports [2,3], we investigated an effect of shortand long-term mild hypothermic conditions on Ig production, cell growth and cell size.